Composite

Part:BBa_K081003

Designed by: Lorenzo Pasotti, Paolo Magni   Group: iGEM08_UNIPV-Pavia   (2008-10-19)

Demux INPUT

This device is a PoPS in -> luxI, lasI out proteins generator with Plas promoter downstream.

It is a module of the following genetic demultiplexer (demux - click [http://en.wikipedia.org/wiki/Multiplexer here] to see what is a demultiplexer):

Pv demux final.png
Pv demuxregistry interactions.png

where:

  • PI and PS are two promoters that can be ACTIVATED by the two generic elements I and S respectively;
  • GOI0 and GOI1 are the two genes of interest that are the outputs of the system.

In particular, K081003 is the Input.

People who want to use the genetic demux have to assemble:

  • K081003 under the desired regulation for Input (for example the generic promoter PI);
  • K081004 under the desired regulation for Selector (for example the generic promoter PS);
  • the desired protein generator for Out0 downstream of K081003 (for example the generic RBS-GOI0-T generator);
  • the desired protein generator for Out1 downstream of K081004 (for example the generic RBS-GOI1-T generator);

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
    Illegal BglII site found at 1354
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 916
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None